黄芪、何首乌、女贞子、菟丝子混合提取物对体外培养毛囊生长的影响及药理作用研究

目的:通过体内外实验探讨黄芪、何首乌、女贞子、菟丝子混合中药提取物对毛囊增殖的影响作用以及其作用机理。方法:通过体外培养的C57BL/6小鼠毛囊器官模型观察不同浓度中药提取物对毛囊生长的影响;采用MTT法测定不同浓度中药提取物对毛乳头增殖的影响;蛋白免疫印迹法(Western Blot)和ELISA检测中药提取物对毛乳头细胞分泌肝细胞生长因子(HGF)的影响。结果:中药提取物能够刺激体外培养的小鼠毛囊的生长,800μg/mL浓度的促进作用最强;160μg/mL中药提取物对毛乳头细胞的增殖作用最强,与米诺地尔、齐墩果酸阳性对照存在显著性差异(P0.05)。而且,药提取物促进了毛乳头细胞分泌HGF。结论:黄芪、何首乌、女贞子、菟丝子混合中药提取物在促进毛发生长中起到重要作用,促进毛乳头细胞增殖和分泌HGF是促进毛囊生长的可能性药理机制。

Effect and Pharmaco-Mechanisms of a Mixed Chinese Herbal Medicine Extract on Hair Follicle Growth in Vitro

Objective:We have investigated here effects of a mixed Chinese herbal medicine extract, comprising of Astragalus mongholicus, Polygonum multiflorum, Ligustrum lucidum, and Semen cuscutae, on hair follicle growth and its mechanism of action through in vitro assays.Methods:Organ model was carried out on cultured hair follicles of C57BL/6 mice in intro to detect the effect of Chinese herbal medicine extract on hair follicle growth; The effect of Chinese herbal medicine extract on the proliferation of dermal papilla cells was measured by methyl thiazolyl terazolium (MTT) method. Western blot and ELISA were used to detect the level of HGF secreted by dermal papilla cells.Results:Chinese herbal medicine extract stimulated the growth of cultured mice hair follicle in vitro. 800 ug/mL had the best promoting effect on the growth of hair follicles. Using follicle dermal papilla cells prepared from mice hair follicle, we showed that Chinese herbal medicine extract promoted maximum proliferation of follicle dermal papilla cells at concentration of 160 ug/mL, which is significantly different from cells treated with minoxidil or oleanolic treated cells (P<0.05). Moreover, Chinese herbal medicine extract enhanced secretion of HGF from dermal papilla cells.Conclusion:Chinese herbal medicine extract, which contains Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Fructus Ligustri Lucidi and Semen Cuscutae, plays a role in hair follicle growth. probably by promoting the proliferation of dermal papilla cells and enhancing secretion of HGF.