兔肝VX2肿瘤射频消融术后残癌中基质金属蛋白酶9表达

目的:探讨兔肝脏VX2肿瘤射频消融术(radio-frequency ablation,RFA)后残余肿瘤组织中基质金属蛋白酶9(MMP-9)表达。方法:超声引导下将VX2肿瘤组织块接种于23只新西兰大白兔肝脏中,造模成功后随机分为5组,对照组(A组,n=3),不行RFA治疗;余20只为实验组行RFA治疗,在超声引导下射频针插入肿瘤偏心位置,展开电极致损毁范围最大为肿瘤总体积的2/3,人为造成残存肿瘤组织。根据治疗结束后不同时间点分为4组:0小时组(B组,n=5)、术后1周组(C组,n=5)、术后2周组(D组,n=5)、术后4周组(E组,n=5),行超声检查结束后处死大白兔,取肿瘤组织采取免疫组化法观察残存肿瘤组织中及未治疗肿瘤组中MMP-9的表达情况。结果:RFA术后0小时、1周、2周、4周残存肿瘤组织中MMP-9的表达均较对照组明显降低(P0.05)。结论:RFA治疗后残存肿瘤细胞中MMP-9水平表达减低。

The Expression of the MMP-9 in Rabbit Liver VX2 Tumor-remnant Tissues after Radio-frequency Ablation

Objective:To investigate the expression of the MMP-9 in rabbit liver VX2 tumor-remnant tissues after radio-frequency ablation treatment (RFA).Methods:Ultrasound-guided the VX2 tumor mass were planted into the liver of 23 New Zealand rabbits, models of rabbit VX2 liver tumor were divided into 5 groups at random, there were 3 rabbits in group A as the control group with no RFA treatment; the remaining 20 rabbits as experimental group with the RFA treatment, Ultrasound-guided the Rf needle was inserted into the location of the liver tumor eccentric, tumor-remnant tissues were remained by opening the electrode to control the maximum damage range of total tumor volume 2/3. 20 rabbits were divided into 4 groups according to their different time after treatment, that was after RFA group (B group), one week after RFA group (C group), two weeks after RFA group (D group), four weeks after RFA group(E group), 5 rats in each group. The rabbits were executed after taking ultrasonographical examination, Immunohistochemistry was used to detect the expression status of MMP-9 in rabbit liver VX2 tumor-remnant tissues after RFA treatment and the untreated tumor tissues.Results:The expression of MMP-9 in tumor-remnant tissues of 0 hour, 1 week, 2 weeks, 4 weeks after RFA were significantly lower than the control group (P<0.05).Conclusion:After RFA the expression of MMP-9 in the remnant tumor cells is decreased.