Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients with systemic lupus erythematosus |
| |
| Authors: | T A Nevzorova V G Vinter O A Konovalova M Kh Salakhov |
| |
| Institution: | (1) Kazan State University, ul. Kremlevskaya 18, 420008 Kazan, Russia |
| |
| Abstract: | Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of patients with systemic lupus erythematosus (SLE). These antibodies displayed a thermostable DNA-hydrolyzing activity and were different in affinity for DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and are active over a wide range of pH. By atomic-force microscopy, three-dimensional images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained with nanometer resolution, and a nonprocessive action mechanism was shown for the DNase activity of antibodies to nDNA. |
| |
| Keywords: | polyclonal catalytic antibodies abzymes systemic lupus erythematosus IgG hydrolysis of DNA atomic-force microscopy |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
