Protoplast regeneration and organogenesis from pea protoplasts |
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Authors: | Renate Lehminger-Mertens Hans-Jörg Jacobsen |
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Institution: | 1. Institut für Genetik der Universit?t Bonn, Kirschalle 1, D-5300, Bonn 1, FRG
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Abstract: | Summary Protoplasts of young Pisum Sativum L. seedlings from 7 different genotypes were isolated and regenerated to the callus stage.
Germinating embryos were cultivated with cotyledons removed, thus avoiding intracellular starch accumulation in donor tissue.
The first lateral shoots provided a source of homogenous meristematic cells which gave rise to sustained protoplast division
and resulted in callus formation within 4, weeks. Root formation occurred on hormone-free medium and shoots developed on medium
containing kinetin, 2iP or zeatin in the third subculture, when subcultured in monthly intervals.
This work was made possible by a joint GFP/BMFT—project to H.-J. Jacobsen (grant A 24/87-ZF). |
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Keywords: | Pisum sativum protoplasts organogenesis agarose droplet culture |
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