The tnpR gene product of TnA is required for transposition immunity |
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| Authors: | L J Wallace J M Ward and M H Richmond |
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| Institution: | (1) Department of Bacteriology, University of Bristol, University Walk, BS8 1TD Bristol, England;(2) Present address: Department of Biochemistry, UMIST, PO Box 88, M60 1QD Manchester, England |
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| Abstract: | Summary A mutant of TnA no longer recognizing immune plasmids has been isolated. The mutation is complemented in trans by a functional tnpR gene. The requirement for wild type tnpR gene product for the establishment of transposition immunity was confirmed by the use of a derivative of transposon Tn3 in which both the tnpA and the tnpR genes are partly deleted. This deleted Tn3 was shown to transpose onto an immune plasmid in the presence of a wild type tnpA gene but not in the presence of both tnpA and tnpR genes. |
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