Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata |
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| Authors: | Ohgawara T Kobayashi S Ohgawara E Uchimiya H Ishii S |
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| Institution: | (1) Bioscience Research Laboratory, Kikkoman Corporation, Noda-shi, 278 Chiba, Japan;(2) Akitsu Branch, Fruit Tree Research Station, Akitsu, 729-24 Hiroshima, Japan;(3) Institute of Biological Sciences, University of Tsukuba, Sakura-mura, 305 Ibaraki, Japan |
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| Abstract: | Summary Somatic hybrid plants of Rutaceae were obtained by protoplast fusion between Citrus sinensis Osb. ( Trovita orange) and Poncirus trifoliata. Protoplasts isolated from embryogenic cells of C. sinensis and from leaves of P. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. Green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. Other morphological characters of these plants were intermediate between both parents. The chromosome number in one of the hybrid plants was 36, which was the sum of C. sinensis (2n=18) and P. trifoliata (2n=18). EcoRI restriction analysis of rDNA confirmed the presence of parental nuclear DNAs in the hybrid. |
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| Keywords: | Citrus Poncirus Embryogenesis rDNA analysis Somatic hybrid |
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