Rho-mediated assembly of stress fibers is differentially regulated in corneal fibroblasts and myofibroblasts |
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Authors: | Anderson Susan DiCesare Lisa Tan Ivan Leung Thomas SundarRaj Nirmala |
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Affiliation: | a Ophthalmology Department, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15213, USA;b Glaxo-IMCB Group, Institute of Molecular and Cell Biology, Singapore 117609, Singapore |
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Abstract: | Corneal keratocytes (stromal cells) are activated to fibroblasts and myofibroblasts during wound healing. Myofibroblast transdifferentiation is accompanied by the expression of α-smooth muscle actin (α-SMA) and the assembly of a robust stress fiber network and larger focal adhesions (FAs). The regulation of the assembly of stress fibers was evaluated in cultured corneal fibroblast and myofibroblast phenotypes. In both cell types, the inhibition of Rho GTPase activity by microinjecting C3 transferase into the cells resulted in the disassembly of stress fibers and FAs. However, the inhibition of the Rho-associated kinases ROKα and ROKβ with their inhibitor, Y27632, or by overexpression of their mutant kinase-dead forms resulted in only a partial loss of the stress fibers and FAs in myofibroblasts but a total loss in fibroblasts. ROK inhibitor-sensitive and -resistant stress fibers in myofibroblasts contained α-SMA, nonmuscle myosin II, tropomyosin, and calponin. The ROK inhibition-resistant stress fibers and FAs were lost upon the overexpression of the dominant-negative form of mDia1 (a mammalian homolog of Drosophila diaphanous protein). These findings indicated that while the assembly of stress fibers in fibroblasts critically involves both ROK and mDia1, in myofibroblasts, the assembly of α-SMA-containing stress fibers also occurs independently of ROK and involves Rho/mDia1. |
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Keywords: | Cornea Corneal stroma Myofibroblast Rho Rho-kinase ROK mDia1 Y27632 |
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