A method for screening bacteria: Aerobically degrading chlorinated short-chain hydrocarbons |
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Authors: | Uwe Strotmann Dr. Robert Röschenthaler |
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Affiliation: | (1) Institute of Microbiology, University of Münster, Corrensstrasse 3, D-4400 Münster, FRG |
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Abstract: | A method for screening of short-chain chlorinated-hydrocarbon-degrading bacteria is described. It uses as a criterium for selection the liberation of protons and change in color of a pH indicator rather than growth on the compound as sole carbon source. The usefulness of indicator plates is demonstrated with several bacteria, known to degrade certain chlorinated hydrocarbons. Bacteria-degrading volatile compounds can be isolated with a medium containing a second carbon source. A correlation between proton liberation and liberation of chloride or disappearance of 2-chloroethanol from the medium is demonstrated as an example. The method should be useful in isolating bacteria for the decontamination of respective commodities. |
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