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Induction of GLUT1 mRNA in response to azide and inhibition of protein synthesis
Authors:Behrooz  Alireza  Ismail-Beigi  Faramarz
Affiliation:(1) Departments of Medicine and Molecular Biology and, Microbiology Case Western Reserve University, Cleveland, Ohio, USA
Abstract:The biochemical behaviour of four commercial strains of Saccharomyces cerevisiae was studied in the presence of calcium ions, acrylamide and bisacrylamide. Calcium ions at a concentration of 300 µM induced an increase of NAD(P)+ reduction in commercial Turkish and American strains, while in Chilean and Brazilian commercial strains, it diminished NAD(P)+ reduction. On the other hand, polyacrylamide monomers (acrylamide and bisacrylamide) induced a decrease of NAD(P)+ reduction in all strains studied in this paper. When membrane potential (DeltaPSgr) and oxygen consumption were measured in the presence of polyacrylamide monomers, a decrease of both was observed in all strains studied.
Keywords:Saccharomyces cerevisiae  NAD(P)H  calcium ions  cells immobilization  oxygen consumption  biotransformation
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