| 刺猬高重复顺序DNA的分子克隆及核苷酸顺序分析 |
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| 引用本文： | 谢清梅,黄秉仁,蔡良琬,林海玉. 刺猬高重复顺序DNA的分子克隆及核苷酸顺序分析[J]. 中国生物化学与分子生物学报, 1993, 9(3): 354-357 |
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| 作者姓名： | 谢清梅 黄秉仁 蔡良琬 林海玉 |
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| 作者单位： | 中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所，中国医学科学院基础医学研究所 北京 100005 进修生，现为医科院动物所工作人员，北京 100005，北京 100005，北京 100005 |
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| 摘 要： | 为了分析比较食虫目高重复顺序的结构特征,我们将食虫目动物刺猬的高重复顺序DNA进行了分离、纯化、分子克隆,并测出了1186bp的核苷酸顺序。经分析发现在碱基组成上它与食虫目另一动物臭鼩有许多相似之处,如:G-C碱基对与A-T碱基对在整个片段中分布很不均衡;片段内部含有许多连续多次重复的短片段等,很可能为食虫目动物所特有。
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| 关 键 词： | 刺猬 高重复顺序 分子克隆 DNA顺序 |
| 收稿时间： | 1993-06-20 |
Cloning and Sequencing of a Highly Repeated DNA Fragment of Hedgehog |
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| Xie,Qingmei Huang,Bingren Cai,Liangwan Lin,Haiyu. Cloning and Sequencing of a Highly Repeated DNA Fragment of Hedgehog[J]. Chinese Journal of Biochemistry and Molecular Biology, 1993, 9(3): 354-357 |
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| Authors: | Xie Qingmei Huang Bingren Cai Liangwan Lin Haiyu |
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| Affiliation: | (Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, 100005 |
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| Abstract: | In order to determine the characteristics of the highly repeated sequence of insectivora, a highly repeated DNA fragment of hedgehog (one Insectivora animal) has been studied by molecular cloning and DNA sequencing. 1186 bp sequence has been obtained. It shows that there are many similarities in highly repeated sequence of Hedgehog and Brown Musk Shrow (another Insectivora animal). These similarities between these two animals may be the characteristics of Insectivora. |
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| Keywords: | Hedgehog Highly Repeated Sequence Molecular Cloning DNA Sequencing |
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