Glucose-dependent insulinotropic peptide stimulates proliferation and TGF-beta release from MG-63 cells |
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Authors: | Zhong Qing Ding Ke-Hong Mulloy Anthony L Bollag Roni J Isales Carlos M |
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Affiliation: | Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA. |
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Abstract: | Glucose-dependent insulinotropic peptide (GIP) is known to modulate alkaline phosphatase activity and collagen type I message in osteoblastic-like cells. GIP effects on cell proliferation are not known. We report that GIP dose dependently stimulated 3H-thymidine incorporation in the osteoblastic-like cell line MG-63. Furthermore, GIP increased message and secretion of transforming growth factor beta (TGF-beta), an agent known to regulate osteoblastic proliferation and differentiation. However, when GIP was added to MG-63 cells concurrently with a TGF-beta neutralizing antibody, there was no effect on 3H-thymidine incorporation in these cells. These data demonstrate that GIP stimulates osteoblastic-like cell proliferation but that this effect is not mediated by TGF-beta. |
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