Marine mammal Brucella isolates with different genomic characteristics display a differential response when infecting human macrophages in culture |
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| Institution: | 1. Scheie Eye Institute, Department of Ophthalmology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania;2. Center for Clinical Epidemiology and Biostatistics, Department of Biostatistics & Epidemiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania;3. Leonard Davis Institute, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania;4. Lake Erie College of Osteopathic Medicine, Bradenton, Florida;1. Department of Ophthalmology, University of Lausanne, Jules Gonin Eye Hospital, Fondation Asile des Aveugles, Lausanne, Switzerland;2. Department of Ophthalmology, Catholic University of Rome, Rome, Italy;3. Centro Italiano Macula, Rome, Italy;4. Nuovo Regina Margherita Hospital, Rome, Italy;5. Inserm, U1138, Team 17, From Physiopathology of Ocular Diseases to Clinical Development, Université Paris Descartes Sorbonne Paris Cité, Centre de Recherche des Cordeliers, Paris, France;1. Radiochemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India;2. Mechanical Metallurgy Division, Bhabha Atomic Research Centre, Mumbai 400085, India;3. Solid State Physics Division, Bhabha Atomic Research Centre, Mumbai 400085, India;4. Radioanalytical Chemistry Division, Bhabha Atomic Research Centre, Mumbai 400085, India;1. Laboratorio de Ecología, Comportamiento y Mamíferos Marinos (LECyMM), División Mastozoología, Museo Argentino de Ciencias Naturales “Bernardino Rivadavia” (MACN-CONICET), Av. Ángel Gallardo 470, C1405DJR Buenos Aires, Argentina;2. Museo Acatushún de Aves y Mamíferos Marinos Australes, Sarmiento 44, 9410 Ushuaia, Tierra del Fuego, Argentina;3. Centro Austral de Investigaciones Científicas (CADIC-CONICET), Houssay 200, V9410BFD Ushuaia, Tierra del Fuego, Argentina;4. Centro de Estudios Biomédicos, Biotecnológicos, Ambientales y Diagnóstico (CEBBAD), Fundación de Historia Natural Félix de Azara, Departamento de Ciencias Naturales y Antropología, Universidad Maimónides, Hidalgo 775, C1405BCK Buenos Aires, Argentina;5. Laboratorio de Análisis por Activación Neutrónica, Centro Atómico Bariloche-Comisión Nacional de Energía Atómica (CAB-CNEA), Av. E. Bustillo 9500, 8400 Bariloche, Argentina |
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| Abstract: | Marine mammal Brucella strains with different genomic characteristics according to distribution of IS711 elements in their genomes were analysed for their intracellular behaviour in human THP-1 macrophage-like cells. Seven different groups of marine mammal strains were identified including a human isolate from New Zealand presumably from marine origin. Entry and intracellular survival of strains representative of these groups in THP-1 human macrophage-like cells were analysed at several times of infection. Three patterns of infection were identified. The Brucella strain isolated from the human case from New Zealand, and two other groups of strains belonging to B. ceti or B. pinnipedialis were able to infect THP-1 macrophage cells to the same extent as the virulent strains B. suis 1330 or B. melitensis 16M. Three other groups of strains belonging to B. ceti or B. pinnipedialis were able to enter the cells as classical virulent strains but were eliminated after 48 h. The last group was composed only of strains isolated from hooded seals (Cystophora cristata) and was even unable to enter and infect THP-1 macrophage cells. Thus, several groups of marine mammal Brucella strains appear to be non-infectious for human macrophages. |
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