Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure |
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| Institution: | 1. InterveXion Therapeutics, LLC, 4301 W. Markham St., #831, Little Rock, AR 72205, USA;2. Department of Pharmacology and Toxicology, College of Medicine, University of Arkansas for Medical Sciences, 4301 W. Markham St., #611, Little Rock, AR 72205, USA;1. Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, Germany;2. Institute for Transfusion Medicine, University of Duisburg-Essen, University Hospital Essen, Germany;3. Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen, Germany |
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| Abstract: | In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28. |
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