Biofilm formation on human airway epithelia by encapsulated Neisseria meningitidis serogroup B |
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| Institution: | 1. Department of Microbiology, 3-401 BSB, The University of Iowa, 51 Newton Rd., Iowa City, IA 52242, USA;2. Central Microscopy Research Facility, 85 EMRB, The University of Iowa, Iowa City, IA 52242, USA;1. CIRI, Centre International de Recherche en Infectiologie, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ Lyon, 69100, Villeurbanne, France;2. Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada;1. PharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, Bonn 53121, Germany;2. Institute of Medical Microbiology, Immunology and Parasitology–Pharmaceutical Microbiology Section, University of Bonn, Bonn 53105, Germany;3. Channing Laboratory, Department of Medicine, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA 02115, USA;4. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China;5. German Centre for Infection Research (DZIF), Partner site Bonn-Cologne, Bonn, Germany;1. Department of Molecular Cell Biology and Immunology, VU University Medical Center, van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands;2. Microbiology, Institute of Biomembranes, Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands;1. Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis;2. Behavioural Science Foundation, Estridge Estate, Saint Kitts and Nevis;3. Gribbles Veterinary Pathology, Christchurch, New Zealand;1. Department of General Internal Medicine, Iizuka Hospital, 3-83 Yoshio-machi, Iizuka, Fukuoka, 820-8505, Japan;2. Department of Infectious Diseases, Iizuka Hospital, 3-83 Yoshio-machi, Iizuka, Fukuoka, 820-8505, Japan;3. Department of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan;4. Department of Orthopedics, Iizuka Hospital, 3-83 Yoshio-machi, Iizuka, Fukuoka, 820-8505, Japan |
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| Abstract: | Neisseria meningitidis is the etiologic agent of meningococcal meningitis. We compared 48-h biofilm formation by N. meningitidis serogroup B strains NMB, MC58, C311 and isogenic mutants defective in capsule formation on SV-40 transformed human bronchial epithelial (HBE) cells in a flow cell. We demonstrated that strains NMB and NMB siaA-D were defective in biofilm formation over glass, and there was a partial rescue of biofilm growth for strain NMB on collagen-coated coverslips at 48 h. We demonstrated all three serogroup B strains form biofilms of statistically equivalent average height on HBE cells as their isogenic capsular mutants. Strain NMB also formed a biofilm of statistically equivalent biomass as the NMB siaA-D mutant on HBE cells at 6 and 48 h. These biofilms are significantly larger than biofilms formed over glass or collagen. Verification that strain NMB expressed capsule in biofilms on HBE cells was demonstrated by staining with 2.2.B, a monoclonal antibody with specificity for the serogroup B capsule. ELISA analysis demonstrated that strains MC58 and C311 also produced capsules during biofilm growth. These findings suggest that encapsulated meningococci can form biofilms on epithelial cells suggesting that biofilm formation may play a role in nasopharyngeal colonization. |
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