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| 1株抗菌植物内生菌EJH-2菌株的分离和鉴定 | |
| 引用本文： | 孙力军,陆兆新,别小妹,孙德坤,杨胜远.1株抗菌植物内生菌EJH-2菌株的分离和鉴定[J].中国微生态学杂志,2006,18(1):23-26. |
| 作者姓名： | 孙力军 陆兆新 别小妹 孙德坤 杨胜远 |
| 作者单位： | 1. 安徽科技学院,工学院,安徽,凤阳,233100;南京农业大学,食品学院,江苏,南京,210095 2. 南京农业大学,食品学院,江苏,南京,210095 3. 安徽科技学院,工学院,安徽,凤阳,233100 |
| 基金项目： | 安徽科技学院校科研和教改项目 |
| 摘 要： | 目的从中药植物金银花的组织中分离到1株具有抗菌活性的植物内生菌EJH-2菌株，并对其进行了分子生物学鉴定。方法通过总DNA提取，PCR扩增，得到1300bp的16S rRNA序列。PCR产物序列通过BLAST软件在NCBI网站中进行同源性比较。通过Bioedit7．0和Treedrawing软件绘制系统发育树。结果EJH-2的16SrRNA序列和数据库中的类多粘芽胞杆菌KCTC 1663菌株的序列的同源性为99．76％。在系统发育树中，EJH-2菌株和多粘类芽胞杆菌在同一分支。结论EJH-2菌株应归属于多粘类芽胞杆菌（Paenibacillus polymyxa）。 |
| 关 键 词： | 内生菌 多粘类芽胞杆菌 鉴定 |
| 文章编号： | 1005-376X（2006）01-0023-04 |
| 收稿时间： | 2006-07-10 |
Isolation and identification of an antimicrobial endophytic strain EJH-2 |
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| SUN Li-jun,LU Zhao-xin,BIE Xiao-mei,SUN De-kun,YANG Sheng-yuan.Isolation and identification of an antimicrobial endophytic strain EJH-2[J].Chinese Journal of Microecology,2006,18(1):23-26. | |
| Authors: | SUN Li-jun LU Zhao-xin BIE Xiao-mei SUN De-kun YANG Sheng-yuan |
| Institution: | 1. School of Industry, Anhui Science and Technology College, Fengyang 233100, China;2. School of Food Science, Nanjing Agricultural University, Nanjing 210095, China |
| Abstract: | Objective One endophyfic strain EJH-2 with strong antimicrobial activity was derived from tissue ot Lonicera japonica . Methods Thunb and identified based on 16S rRNA gene sequences. The sequence fragment of 1 300 bp 16S ribosomal RNA was amplified from total DNA of strain EJH-2 by PCR. The sequence analysis was down by the application of BLAST on the websites of NCBI and the phylogenctic tree was drawn by Bioedit 7.0 and Treedrawing. Results EJH-2 shared 99.76% 16S rRNA sequence homology with Paenibacillus polymyxa KCTC 1663. EJH-2 and Paenibacillus polymyxa were in same branch of phylogenetic trees. Conclusion The strain EJH-2 belongs to Paenibacillus polymyxa. |
| Keywords: | 16S Rrna |
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