Quick DNA preparation for direct PCR in marine invertebrates and fishes |
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| Authors: | Reynold Peter Siby Philip Allen Benziger |
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| Institution: | (1) Marine Biotechnology Division, Central Marine Fisheries Research Institute, Ernakulam, Kerala, 682018, India;(2) Department of Zoology, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, India;(3) Zebra Fish Genomics Lab, Institute of Genomics and Integrative Biology, New Delhi, India |
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| Abstract: | A modified protocol was developed for extracting DNA and direct PCR from tissues of marine invertebrates and finfishes. Protocol
represented combination of classical lysis using a modified buffer system comprising KCl, Tris buffer and MgCl2. The DNA obtained was quantified and tested by mtDNA-PCR and RAPD. Test results indicated usefulness of this method in studies
involving screening of large numbers of samples, such as evolutionary, forensic and population studies. |
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| Keywords: | Marine invertebrates Fishes DNA extraction Direct-PCR Population genetics Molecular taxonomy |
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