The Drosophila ACP65A cuticle gene: deletion scanning analysis of cis-regulatory sequences and regulation by DHR38 |
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Authors: | Bruey-Sedano N Alabouvette J Lestradet M Hong L Girard A Gervasio E Quennedey B Charles J P |
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Institution: | UMR CNRS 5548 Développement-Communication Chimique, Université de Bourgogne, Faculté des Sciences Gabriel, Dijon, France. |
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Abstract: | The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between -594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene. |
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Keywords: | Drosophila cuticle ecdysteroids juvenile hormones NGFI‐B DHR38 ACP65A epidermis |
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