Analysis of biochemical markers of MCF-7 cell apoptosis induced by a recombinant analogue of lactaptin |
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| Authors: | A S Fomin O A Koval D V Semenov M O Potapenko E V Kuligina Yu Ya Kit V A Richter |
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| Institution: | 1.Institute of Chemical Biology and Fundamental Medicine,Russian Academy of Sciences, Siberian Branch,Novosibirsk,Russia;2.Institute of Cell Biology,National Academy of Sciences,Lviv,Ukraine |
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| Abstract: | Earlier, a pro-apoptotic peptide lactaptin was isolated from human milk and characterized and its recombinant analogue RL2
was generated. Both peptides were demonstrated to be capable of apoptotic cell death induction in human breast adenocarcinoma
MCF-7 cells. In the work, biochemical markers of RL2-induced MCF-7 apoptosis are analyzed. Activation of initiator and effector
caspases, as well as apoptotic changes in mitochondrial membrane potential and cytoplasm membrane composition in cells treated
with RL2, were analyzed using flow cytometry and Western blot techniques. MCF-7 cell death induced by RL2 was found to be
associated with phosphatidylserine exposure on the surface of the plasma membrane. Also, RL2 was demonstrated to induce dissipation
of the mitochondrial membrane potential and activation of initiator caspases 8 and 9 and an effector caspase 7. |
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