Production of neutral and alkaline extracellular proteases by the thermophilic fungus, Scytalidium thermophilum, grown on microcrystalline cellulose |
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Authors: | ? Hasbay ?frij ZB Ögel |
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Institution: | (1) Department of Food Engineering, Middle East Technical University, Ankara, 06531, Turkey |
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Abstract: | Extracellular proteases produced by Scytalidium thermophilum, grown on microcrystalline cellulose, were most active at pH 6.5–8 and 37–45 °C when incubated for 60 min. Highest protease activity was at day 3 where endoglucanase activity was low. Protease activity measurements with and without the protease inhibitors, p-chloromercuribenzoate, PMSF, antipain, E-64, EDTA and pepstatin A, suggest production of thiol-containing serine protease and serine proteases. Endoglucanase and Avicel-adsorbable endoglucanase activity in culture medium was not significantly affected by protease inhibitors. |
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Keywords: | endoglucanase protease Scytalidium thermophilum thermophilic fungus |
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