Regeneration and micrografting of lentil shoots |
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Authors: | Anju Gulati Pat Schryer Alan McHughen |
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Affiliation: | (1) Crop Development Centre, University of Saskatchewan, 51 Campus Drive, SK S7N5A8 Saskatoon, Canada |
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Abstract: | Summary A protocol for regeneration and micrografting of shoots of lentil (Lens culinaris Medik) was developed. Multiple shoots (4–5) were regenerated from cotyledonary node explants on Murashige and Skoog (MS) medium containing 8.8 μM 6-benzylaminopurine. In vitro regenerated shoots were micrografted on rootstocks with 96% efficiency. The successful grafts were transplanted to pots in Redi-earthTM, hardened off and were grown to maturity with 100% success. The success of the micrografting was independent of the nature and concentration of growth regulator used in shoot initiation medium and the time period for induction of shoots. The protocol was successful with several cultivars of lentil. The advantages of micrografting over in vitro rooting are discussed. |
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Keywords: | in vitro shoot regeneration lentil micrografting rooting |
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