| CoCl2预处理对大鼠海马神经元急性低氧后Na+、K+电流的影响 |
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| 引用本文： | Zhao T,Liu W,Wu LY,Ding AS,Wang FZ,Fan M. CoCl2预处理对大鼠海马神经元急性低氧后Na+、K+电流的影响[J]. 中国应用生理学杂志, 2003, 19(3): 250-252 |
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| 作者姓名： | Zhao T Liu W Wu LY Ding AS Wang FZ Fan M |
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| 作者单位： | 1. 军事医学科学院基础医学研究所神经生物研究室,北京,100850
2. 解放军第88医院,山东,泰安,271000 |
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| 基金项目： | 国家自然科学基金重点资助项目 ( 39730 190 ) |
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| 摘 要： | 目的：观察氯化钴(COCl2)预处理对急性低氧后海马神经元电压门控性Na^ 、K^ 电流的影响。方法：原代培养大鼠海马神经元,分为COCl2预处理和非处理组,采用膜片钳全细胞记录技术,检测急性低氧后海马神经元钠电流(INa)、钾电流(Ik)的变化。结果：急性低氧后,海马神经元INa、Ik电流幅度明显降低,INa阈值右移,而经CoCl2预处理的海马神经元INa、Ik电流的降低幅度明显减轻。结论：COCl2预处理减轻急性低氧所致的INa、Ik电流变化,对神经元有明显的保护作用。
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| 关 键 词： | COCl2预处理 大鼠 海马神经元 急性低氧 Na^＋、K^＋电流 膜片钳 |
| 文章编号： | 1000-6834(2003)03-0250-03 |
| 修稿时间： | 2002-06-10 |
Effect of CoCl2 pretreatment on Na + and K+ currents of the rat hippocampal neurons after acute hypoxia |
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| Zhao Tong,Liu Wei,Wu Li-Ying,Ding Ai-Shi,Wang Fu-Zhuang,Fan Ming. Effect of CoCl2 pretreatment on Na + and K+ currents of the rat hippocampal neurons after acute hypoxia[J]. Chinese journal of applied physiology, 2003, 19(3): 250-252 |
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| Authors: | Zhao Tong Liu Wei Wu Li-Ying Ding Ai-Shi Wang Fu-Zhuang Fan Ming |
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| Affiliation: | ZHAO Tong 1,LIU Wei 2,WU Li ying 1,DING Ai shi 1,WANG Fu zhuang 1,FAN Ming 1 |
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| Abstract: | Aim: To study effect of CoCl 2 pretreatment on the voltage gated Na + and K + currents of the rat hippocampal neurons after acute hypoxia. Methods: Primarily cultured hippocampal neurons were divided into CoCl 2 pretreated and non pretreated groups. Patch clamp whole cell recording technique was used to examine Na + and K + currents of the hippocampal neurons. Results: After acute hypoxia, I Na and I K of the hippocampal neurons were significantly decreased and the threshold of I Na was right shifted. Pretreatment of the neurons with CoCl 2 inhibited the reduction of I Na and I K. Conclusion: CoCl 2 pretreatment alleviates the acute hypoxia induced changes of I Na and I K, which may be one of the mechanisms for the protective effect of CoCl 2 on neurons. |
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| Keywords: | CoCl 2 pretreatment hypoxia hippocampal neurons patch clamp |
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