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Regional distribution of DNA and RNA in rat brain: A sensitive determination using high-performance liquid chromatography with electrochemical detection |
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| Authors: | Tatsuo Nakahara Makoto Hirano Takashi Matsumoto Toshihide Kuroki Yoshinori Tatebayashi Tetsuyuki Tsutsumi Kouji Nishiyama Hiroaki Ooboshi Kaoru Nakamura Hiroshi Yao Akio Shiraishi Michinori Waki Hideyuki Uchimura |
| Institution: | (1) Department of Chemistry, Faculty of Science, Kyushu University, Hakozaki, Higashiku, 812 Fukuoka, Japan;(2) Center for Emotional and Behavioral Disorders, Hizen National Mental Hospital, Kanzaki, 842-01 Saga, Japan;(3) Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashiku, 812 Fukuoka, Japan |
| Abstract: | DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control. |
| Keywords: | DNA RNA central nervous system electrochemical detection HPLC |
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