The alkaline cleavage and borohydride reduction of cartilage proteoglycan |
| |
Authors: | H. Clem Robinson and John J. Hopwood |
| |
Affiliation: | Department of Biochemistry, Monash University, Clayton, Vic. 3168, Australia |
| |
Abstract: | A method for the rapid isolation and purification of proteoglycan by using neutral solutions of LiBr for extraction and density-gradient centrifugation is described. The effect of 0.5m-KOH on isolated proteoglycan has been studied by using NaB(3)H(4) to reduce and label the chondroitin sulphate chains released. This study has established: (a) that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali-labile bonds between xylose and serine; (b) that random degradation of the chondroitin sulphate chains does not occur to any significant extent; (c) that the method is convenient for the determination of polysaccharide number-average molecular weights. |
| |
Keywords: | |
|
|