Differences between ranamargarin and other tachykinins in the stimulation of ion transport in frog skin |
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Authors: | Claudio Lippe Vito Bellantuono Giuseppe Cassano Angelo Quaranta Concetta Ardizzone |
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Affiliation: | a Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari -Via Amendola 165/A -70126 Bari, Italy b Dipartimento Farmaco-Biologico-Università di Bari -Via Amendola 165/A -70126 Bari, Italy |
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Abstract: | In frog skin, tachykinins stimulate ion transport by interaction with NK1-like receptors. The structural requirements of the peptide are the presence of the C-terminal sequence Phe-X-Gly-Leu-Met-NH2 and at least one Pro residue in the N-terminal sequence. In this paper, we demonstrate that the C-terminal amino acid must be amidated but it can be different from Met, and that the sequence cannot be longer or shorter than 11–12 amino acids. Unexpectedly, Ranamargarin (14 amino acids, no Pro residue) increased the short circuit current value by 48 ± 0.3%. On the basis of considerable experimental evidence, we suggest that Ranamargarin interacts with a receptor different from those of other tachykinins. |
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Keywords: | Ranamargarin Tachykinins Ion Transport Skin Rana esculenta |
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