Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant,anti-inflammatory,and antitumoral effects |
| |
Authors: | Luiza Sheyla E P Will Castro Thuane S Pinheiro Allisson J G Castro Celina M P G Dore Naissandra B da Silva Monique Gabriela das C Faustino Alves Marilia S Nascimento Santos Edda Lisboa Leite |
| |
Institution: | 1. Laboratory of Glicobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, Lagoa Nova, Natal, Rio Grande do Norte, Brazil 2. Department of Histology, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, Lagoa Nova, Natal, Rio Grande do Norte, Brazil 3. Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59082-970, Natal, Rio Grande do Norte, Brazil
|
| |
Abstract: | This study analyzed sulfated polysaccharides, such as fucans, from the brown alga Lobophora variegata to verify their antioxidant activity in vitro, antitumoral effect on human colon adenocarcinoma cell line HT-29, and anti-inflammatory activity. Sulfated polysaccharide fractions containing fucans were obtained after fractionation with increasing volumes (v) of acetone (0.3–2.0 v). The polysaccharide was eluted with 1.5 v of acetone and named F1.5. The results showed that F1.5 contained a high yield. Chemical and structure analyses were performed by infrared spectroscopy, electrophoresis in agarose gel, and chemical dosages (sugar, protein, phenolic compounds, and sulfate). We observed that this sulfated polysaccharide had antioxidant activity and antitumoral effect. Anti-inflammatory activity in vivo of F1.5 was observed in the croton oil mouse-ear model at 75 mg kg-1. The results were correlated with histopathological analysis. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|