Automatic computation of enzyme kinetics by HPLC |
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| Authors: | Mauri, Pier Luigi Bitonti, Maria Pace, Mario Pietta, Pier Giorgio Agnellini, Dario |
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| Affiliation: | Dipartimento di Scienze e Tecnologie Biomediche, Sez. Chimica Organica, University of Milano via G.Celoria 2, 20133 Milano
1Ing, C. Olivetti & Co. S.p.A. Ivrea, Italy |
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| Abstract: | Enzyme activity can be easily measured by HPLC using tracesof the product itself as an internal standard. Our procedureinvolved the development of an equation using the experimentaldata obtained in the kinetic assay. The entire procedure canthus be automated and a computer program is presented here forfacilitating the assay and saving time. The determination ofthe activity of NAD kinase is reported as an example. Received on March 15, 1990; accepted on May 17, 1990 |
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