克鲁氏假丝酵母在高渗环境中海藻糖和胞内甘油积累的研究

Brown1]在1976年提出了相容溶质(Compatible solutes)的概念,尽管有关它们功能的确切机制尚不是非常清楚,但是通常它们被认为是具有渗透调节作用和对细胞中生物活性物质具保护功能的物质.海藻糖和甘油在这方面所表现出的特殊功能已被国内外广泛关注2].Brown3]和Crowe4]还分别报道了甘油和海藻糖在保护胞内可溶性酶和细胞膜稳定性方面的功能.Crowe5]在研究几种不同碳水化合物对动物肌细胞的保护功能时发现,海藻糖和甘油都在不同程度上表现出这种特性.关于酵母细胞在加盐培养基中的生长代谢情况Kuniho Nakata6]和Sukesh 7]分别进行了报道,发现酵母细胞内有海藻糖的积累,并且海藻糖的量与细胞对外界不利环境的耐受性有密切关系.

The Metabolism of Trehalose and Intracellular Glycerol in Candida krusei Responsing to High Osmosis

The trehalose and intracellular glycerol contents of osmotolerant yeast Candida krusei cells rapidly increased in response to the medium containing NaCl. Addition of NaCl at early exponential phase or steady phase increased trehalose content only, but the content of extracellular glycerol increased gradually. Development of resistance was accompanied by accumulation of trehalose and was apparently unrelated to intracellular glycerol content which was always low under these conditions. There seems to be an overlap function between trehalose and glycerol in Candida krusei cells in response to the osmotic conditions.