Phylogeny of the genus Azospirillum based on 16S rDNA sequence |
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| Authors: | Renato Fani Claudio Bandi Marco Bazzicalupo Maria Teresa Ceccherini Silvia Fancelli Enzo Gallori Letizia Gerace Annamaria Grifoni Nerino Miclaus Giuseppe Damiani |
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| Affiliation: | Dipartimento di Biologia Animale e Genetica "Leo Pardi ", Universitàdi Firenze, Via Romana 17,1–50125 Florence, Italy; Istituto di Patologia Generale Veterinaria, Universitàdi Milano, Via Celoria 10,1–20133 Milan, Italy; Istituto Sperimentale per lo Studio e la Difesa del Suolo P.zza D'Azeglio 30,1–50121 Florence, Italy; IDVGA-CNR, Via Celoria 10,1–20133 Milan, Italy |
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| Abstract: | Abstract The 16S rDNA of 17 strains of Azospirillum , 14 assigned to one of the known species A. amazonense A. brasilense A. halopraeferens A. irakense and A. lipoferum , and the other three of uncertain taxonomic position, was sequenced after polymerase chain reaction amplification and analysed in order to investigate the phylogenetic relationships at the intra-generic and super-generic level. The phylogenetic analysis confirms that the genus Azospirillum constitutes a phylogenetically separate entity within the a subclass of Proteobacteria and that the five species are well defined. A. brasilense and A. lipoferum are closely related species and form one cluster together with A. halopraeferens ; the pair of species A. amazonense and A. irakense forms a second cluster in which Rhodospirillum centenum is also placed. |
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| Keywords: | Azospirillum Phylogeny 16S ribosomal DNA Polymerase chain reaction |
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