Host-controlled modification and restriction in Bacillus subtilis: Bsu 168-system and BsuR-system in B. subtilis 168. |
| |
Authors: | Shukuko Ikawa Takehiko Shibata Tadahiko Ando Hiuga Saito |
| |
Affiliation: | (1) Laboratory of Microbiology, The Institute of Physical and Chemical Research, 351 Wako-shi, Saitama, Japan;(2) The Institute of Applied Microbiology, Tokyo University, Bunkyo-Ku, 113 Tokyo, Japan |
| |
Abstract: | Summary A Bsu168-specific restriction deficient (r168-) mutant of Bacillus subtilis Marburg 168 was transformed to be BsuR-specific restriction proficient (rR+) with B. subtilis R DNA as efficiently as the Bsu168-specific restriction proficient (r168+) parental strain (hsrM+, hsdR-).We constructed rR+mR+r168+m168+strain (ISMR 4), rR+mR+r168-m168+strain (ISR 11) and rR+mR+r168-m168-strain (ISR 6) from strain 101 (r168+m168+), strain 1012 (r168-m168+) and strain RM125 (r168-m168-), respectively by transformation with B. subtilis R DNA, and tested their restriction and modification activities on phage 105C. The results show that the sites recognized by Bsu168-specific restriction and modification enzymes and the sites recognized by BsuR-specific ones are not overlapping.We conclude that the Bsu168-modification and restriction system and the BsuR-modification and restriction system are controlled independently by two distinct sets of genes in the rR+mR+transformant of r168+m168+strain B. subtilis 168. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|