Host-controlled modification and restriction in Bacillus subtilis: Bsu 168-system and BsuR-system in B. subtilis 168. |
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Authors: | Shukuko Ikawa Takehiko Shibata Tadahiko Ando and Hiuga Saito |
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Institution: | (1) Laboratory of Microbiology, The Institute of Physical and Chemical Research, 351 Wako-shi, Saitama, Japan;(2) The Institute of Applied Microbiology, Tokyo University, Bunkyo-Ku, 113 Tokyo, Japan |
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Abstract: | Summary A Bsu168-specific restriction deficient (r
168
-
) mutant of Bacillus subtilis Marburg 168 was transformed to be BsuR-specific restriction proficient (r
R
+
) with B. subtilis R DNA as efficiently as the Bsu168-specific restriction proficient (r
168
+
) parental strain (hsrM
+, hsdR
-).We constructed r
R
+
m
R
+
r
168
+
m
168
+
strain (ISMR 4), r
R
+
m
R
+
r
168
-
m
168
+
strain (ISR 11) and r
R
+
m
R
+
r
168
-
m
168
-
strain (ISR 6) from strain 101 (r
168
+
m
168
+
), strain 1012 (r
168
-
m
168
+
) and strain RM125 (r
168
-
m
168
-
), respectively by transformation with B. subtilis R DNA, and tested their restriction and modification activities on phage 105C. The results show that the sites recognized by Bsu168-specific restriction and modification enzymes and the sites recognized by BsuR-specific ones are not overlapping.We conclude that the Bsu168-modification and restriction system and the BsuR-modification and restriction system are controlled independently by two distinct sets of genes in the r
R
+
m
R
+
transformant of r
168
+
m
168
+
strain B. subtilis 168. |
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Keywords: | |
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