The impenetrability of 5-(N-hexadecanoyl)aminofluoroscein through endothelial cell monolayers is dependent upon its solution properties, not the presence of tight junctions. |
| |
Authors: | D B Stolz M G Mahoney B S Jacobson |
| |
Institution: | Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst 01003. |
| |
Abstract: | The solution properties of two fluorescent lipophilic analogues were examined in conjunction with their ability to penetrate the tight junctions of bovine aortic endothelial cell monolayers. 5-(N-dodecanoyl)aminofluoroscein was shown to label both the apical and basolateral plasma membrane domains of confluent monolayers at 4 degrees C and pH 7.3, but 5-(N-hexadecanoyl)aminofluoroscein was shown to label only the apical membrane domain. When used under more soluble conditions at 20 degrees C and pH 8.5, both probes labeled apical and basolateral plasma membrane domains more equally. This indicates that solubility conditions, and not tight junctions, dictate the penetration of 5-(N-hexadecanoyl)aminofluoroscein from the apical to the basolateral plasma membrane domain. |
| |
Keywords: | |
|
|