Estimation of fermentation biomass concentration by measuring oxygen uptake off-line with an oxygen electrode |
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Authors: | Detlef Siegmund Hans Diekmann |
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Affiliation: | (1) Institut für Mikrobiologie, Universität Hannover, Schneiderberg 50, D-3000 Hannover 1, Federal Republic of Germany;(2) Present address: Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstrasse 40, D-2300 Kiel, Federal Republic of Germany |
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Abstract: | Summary An indirect method for biomass determination was developed for aerobic cultivation in media containing solid substrates. This method involved off-line estimation of oxygen uptake with an oxygen electrode using a calibration factor and was applied to penicillin fermentation with Penicillium chrysogenum. The volumetric oxygen uptake rate was independent of the amount of sample sealed in the measuring cell. Storage of the sample for a period up to 2.5 h also had no influence on the rate of O2 uptake. The specific O2 uptake rate at 70 to 75 h fermentation time was used as the calibration factor to calculate the respiration-active part of the biomass. Specific penicillin V formation rates related to net dry weight, respiration-active biomass (XA)and RNA content were compared. Only the penicillin V production rate related to XAshowed a similar behaviour as the penicillin V production rate related to RNA content. For this reason, it is assumed that XA,like RNA content, is more closely related to the metabolic state of the cells than the net dry weight. This method, therefore, provides a more suitable reference parameter for fermentation control than the generally used net dry weight.Offprint requests to: D. Siegmund |
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