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Simultaneous determination of ascorbic acid and dehydroascorbic acid in fish tissues by high-performance liquid chromatography
Authors:Takafumi Ito   Hisashi Murata   Yoshihide Yasui   Morimasa Matsui   Tadashi Sakai  Kiyoshi Yamauchi
Affiliation:1. Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA;2. 72 Marina Lakes Drive, Richmond, CA 94804, USA;3. Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA;1. Aix Marseille Université, Université de Toulon, CNRS/INSU/IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, France;2. Biogeochemistry Research Centre, School of Geography, Earth and Environmental Sciences, University of Plymouth, Drake Circus, Plymouth, Devon PL4 8AA, UK;3. Australian Centre for Ancient DNA, School of Biological Sciences, Faculty of Sciences, The University of Adelaide, Adelaide, SA 5005, Australia;4. Department of Geology, Colgate University, Hamilton, NY 13346, USA;5. Research School of Earth Sciences, The Australian National University, Acton, Australian Capital Territory 2601, Australia
Abstract:An high-performance liquid chromatographic method with post-column derivatization has been developed for the simultaneous determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in fish tissues. Extracted AA and DHAA were separated by a Shim-pack SCR-101H column within 20 min, reacted with sodium hydroxide containing sodium borohydride and monitored at 300 nm. The detection limits for both AA and DHAA were 0.1 μg/ml.
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