| 定向进化人α型干扰素基因家族的研究 |
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| 引用本文： | 段招军,王红艳,李武平,吕宏亮,赵军,魏开坤,马学军,侯云德. 定向进化人α型干扰素基因家族的研究[J]. 病毒学报, 2003, 19(4): 301-305 |
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| 作者姓名： | 段招军 王红艳 李武平 吕宏亮 赵军 魏开坤 马学军 侯云德 |
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| 作者单位： | 中国疾病预防控制中心,病毒病预防控制所,病毒基因工程国家重点实验室,北京,100052 |
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| 摘 要： | 应用DNA改组(DNAshuffling)技术重组了12种人α型干扰素基因,并结合噬菌体表面呈现(phagedisplay)技术构建了噬菌体干扰素改组文库,采用简便的、功能性的定向竞争筛选方法,获得了比活达1 0×109IU/mg的新型α型基因工程复合干扰素,是目前国际上已投产的α2型干扰素的5倍,具有生产应用前景,上述策略也为其它细胞因子或酶的改造提供了借鉴方法。
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| 关 键 词： | DNA改组 噬菌体表面呈现技术 干扰素 表达 纯化 |
| 文章编号： | 1000-8721(2003)04-0301-05 |
Study on the Directed Evolution of Interferon Family |
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| DUAN Zhao-jun,WANG Hong-yan,LI Wu- ping,LV Hong-liang,ZHAO Jun,WEI Ka i-kun,MA Xue-jun,HOU Yun-deational Institute for Viral Disea se Control and Prevention,China CD C,Beijing ,China). Study on the Directed Evolution of Interferon Family[J]. Chinese journal of virology, 2003, 19(4): 301-305 |
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| Authors: | DUAN Zhao-jun WANG Hong-yan LI Wu- ping LV Hong-liang ZHAO Jun WEI Ka i-kun MA Xue-jun HOU Yun-deational Institute for Viral Disea se Control Prevention China CD C Beijing China) |
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| Affiliation: | DUAN Zhao-jun,WANG Hong-yan,LI Wu- ping,LV Hong-liang,ZHAO Jun,WEI Ka i-kun,MA Xue-jun,HOU Yun-deational Institute for Viral Disea se Control and Prevention,China CD C,Beijing 100052,China) |
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| Abstract: | This is the report of dir ected evolution of Human Interfero n Alpha Genes using DNA shuffling combined with phage display techno logy and cell-based competitive sc reening methods.The novel interfer on alpha hybrid with improved bioa ctivity was obtained and shows a g reat prospect in production.This s trategy also offers some informati on on the way of directed evolutio n of other cytokines or proteins. |
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| Keywords: | DNA shuffling phage disp lay interferon expression purifica tion |
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