Genotype-dependent whole plant regeneration from protoplasts of red clover (Trifolium pratense L.) |
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| Authors: | J. R. Myers J. W. Grosser N. L. Taylor G. B. Collins |
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| Affiliation: | (1) Department of Agronomy, University of Kentucky, 40546 Lexington, KY, USA;(2) Present address: Research and Extension Center, University of Idaho, Route 1 3793 N. 3600 E., 83341 Kimberley, ID, USA;(3) Present address: Citrus Research and Education Center, University of Florida, Experiment Station Road, 700 Lake Alfred, FL 33850, USA |
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| Abstract: | Protoplasts are useful for subcellular studies, in vitro selection, somatic hybridization and transformation. Whole plant regeneration from protoplasts is a prerequisite to producing altered crop plants using these methods. Whole plant regeneration was achieved from leaf- and suspension culture-derived protoplasts of T. pratense. Regeneration was most dependent upon identifying genotypes with genetic capacity to regenerate. Additional factors that were used to select genotypes, but which proved to be less important, were a high rate of cell growth in culture and a high plating efficiency of protoplasts. One genotype was identified which had a regeneration response equivalent to that of T. rubens and which regenerated from both leaf- and suspension culture-derived protoplasts.Research supported by USDA/CRGO Grant No. 81 CRCR-1-0613 |
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| Keywords: | somatic embryogenesis plant regeneration protoplasts Trifolium pratense red clover protoclonal variation |
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