| 螺旋藻别藻蓝蛋白的纯化、理化特性与结晶 |
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| 引用本文： | 张少斌,刘慧,刘国琴,商树田. 螺旋藻别藻蓝蛋白的纯化、理化特性与结晶[J]. 微生物学通报, 2006, 33(5): 31-34 |
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| 作者姓名： | 张少斌 刘慧 刘国琴 商树田 |
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| 作者单位： | 1. 沈阳农业大学生物科学技术学院,沈阳,110161
2. 中国农业大学植物生理学与生物化学国家重点实验室,北京,100094 |
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| 基金项目： | 辽宁省教育厅项目（No.20040331）;沈阳农业大学青年教师基金（No.200401） |
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| 摘 要： | 硫酸铵分级沉淀结合多种层析技术，从螺旋藻（SP—Dz）中纯化到电泳纯别藻蓝蛋白（Allophycocyanin，APC），纯度（A650／A280）达4．83。APC在30min内的荧光扫描曲线为直线；30min连续光照其相对荧光强度仍为原来的98％以上，其抗荧光淬灭能力强于同样条件下的藻蓝蛋白（PC）及荧光素TRITC。用悬滴气相扩散法培养获得了APC晶体。
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| 关 键 词： | 钝顶螺旋藻 别藻蓝蛋白 光谱 晶体 |
| 文章编号： | 0253-2654（2006）05-0031-04 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2006-02-23 |
Purification, Physio-chemical Property and Crystallization of APC from Spirulina |
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| ZHANG Shao-Bin,LIU Hui,LIU Guo-Qin,SHANG Shu-Tian. Purification, Physio-chemical Property and Crystallization of APC from Spirulina[J]. Microbiology China, 2006, 33(5): 31-34 |
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| Authors: | ZHANG Shao-Bin LIU Hui LIU Guo-Qin SHANG Shu-Tian |
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| Affiliation: | 1.Biological Science and Technology College, Shenyang Agricultural University, Shenyang 110161;2.State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094 |
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| Abstract: | APC of electrophoresis-purity was obtained from Spirulina using ammonium sulfate grade precipitation followed with several gel chromatography, and the purity (A_ 650 /A_ 280 ) of which attained to 4.83. The fluorescence scan curve of APC was a direct line in 30 min; After exposed in light for 30 min, the relative fluorescence strength of APC preserved above 98 percent of that as begin. APC has a stronger anti-fluorescence quenching than PC or TRITC under the same condition. APC crystals were grown by hanging drop vapor diffusion method. |
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| Keywords: | Spirulina platensis Allophycocyanin Spectrum Crystal |
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