应用于染色体步移的PCR扩增技术的研究进展

各种建立在PCR基础上染色体步移的方法能够根据已知的基因序列得到侧翼的基因序列。染色体步移技术主要应用于克隆启动子、步查获得新物种中基因的非保守区域、鉴定T-DNA或转座子的插入位点、染色体测序工作中的空隙填补，从而获得完整的基因组序列等方面。其方法主要有3种：反向PCR的方法，连接法介导的PCR的方法以及特异引物PCR的方法。文章就各种方法进行举例说明并加以分析比较。

Progress of the PCR Amplification Techniques for Chromosome Walking

Various PCR-based methods are available for chromosome walking from a known sequence to an unknown region.It is promising in genome-related research for the following experiments: promoter cloning,obtaining non-conservative parts of genes in new species,identification of T-DNA or transposon insertion sites and filling in gaps or unknown chromosome regions in genome sequencing.These methods consisted of three types: inverse PCR,ligation-mediated PCR and specific primer PCR.In this review,we illustrated and compared the current techniques.