Evidence for heme oxygenase-1 association with caveolin-1 and -2 in mouse mesangial cells |
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| Authors: | Jung Nam-Hee Kim Hong Pyo Kim Bum-Rae Cha Seok Ho Kim Gyeong A Ha Hunjoo Na Young Eun Cha Young-Nam |
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| Affiliation: | Department of Pharmacology and Toxicology, College of Medicine, Inha University, Incheon, Korea. |
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| Abstract: | The interaction of heme oxygenase-1 (HO-1) and caveolin in the cultured mouse mesangial cells (MMC) was investigated. In normal MMCs, high levels of caveolin-2 and low level of caveolin-1 at mRNA and protein level were observed without any detectable expression of caveolin-3. Upon treating the MMCs either with cadmium (Cd) or spermine NONOate (SPER/NO), expression of HO-1 mRNA and protein was increased. Caveolae rich membranous fractions from the MMCs treated with Cd or SPER/NO contained both HO-1 and caveolin-1 or caveolin-2. The experiments of immuno-precipitation showed complex formation between the HO-1 and caveolin-1 or caveolin-2 in the Cd treated MMCs. Confocal microscopic results also support co-localization of HO-1 and caveolin-1 or caveolin-2 at the plasma membrane. Co-localization of caveolins with HO-1 in caveolae suggested that caveolin could also play an important role in regulating the function of HO-1. |
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