Haloacetol phosphates as affinity labels for methylglyoxal synthase |
| |
Authors: | P M Yuan R W Gracy F C Hartman |
| |
Affiliation: | 1. Departments of Chemistry and Basic Health Sciences, North Texas State University, Denton, Texas 76203, USA;2. Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, USA |
| |
Abstract: | 3-Bromo- and 3-iodoacetol phosphates irreversibly inactivate methylglyoxal synthase. The substrate, dihydroxyacetone phosphate, and inorganic phosphate protect against the inhibition. Although the 3-chloro derivative does not inactivate the enzyme, it is a competitive inhibitor. Reduction of the enzyme-inactivator complex with []-NaBH4 indicates the incorporation of four haloacetol phosphates per mole of enzyme. These studies suggest the bromo- and iodoacetol phosphates inactivate the enzyme by reacting with a nucleophilic group located in the active center. |
| |
Keywords: | To whom correspondence should be addressed. |
本文献已被 ScienceDirect 等数据库收录! |
|