Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry |
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| Authors: | Michael W. Wolff Hélène G. Bazin Robert J. Lindhardt |
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| Affiliation: | (1) Department of Chemistry, Division of Medicinal & Natural Products Chemistry, and Department of Chemical & Biochemical Engineering, The University of Iowa, PHAR-S328, Iowa City, IA 52242, USA |
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| Abstract: | Neutral oligosaccharides were fluorescently conjugated with 7-amino-1, 3-naphthalenedisulfonic acid. A mixture of fluorescently labeled chitobiose, chitotriose, and chitotetrose were successfully separated by preparative capillary electrophoresis (CE) and the individual components characterized by electrospray ionization-mass spectrometry (ESI-MS). By combining fluorescent labeling with CE, the use of highly specific exoglycosidases and ESI-MS, a more structurally complex N-linked glycan was analyzed. |
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| Keywords: | electrospray mass spectrometry exoglycosidase neutral oligosaccharide preparative capillary electrophoresis |
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