Effect of acetoacetyl coa thiolase amplification on sterol synthesis in the yeasts S. cerevisiae and S. uvarum |
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| Authors: | S Dequin F Boutelet M Servouse F Karst |
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| Institution: | (1) TEPRAL S.A., 20 rue de Jacob, 67200 Strasbourg, France;(2) Laboratoire de Biochimie et Genetique des Microorganismes, J.E. CNRS 034620 Université de Poitiers, 40 avenue du recteur Pineau, F-86022 Poitiers cedex, France;(3) Present address: Centre National de Transfusion Sanguine, 6 rue Alexandre Cabanel, 75015 Paris, France |
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| Abstract: | Summary The ERG10 gene was amplified inS. cerevisiae and inS. uvarum, in order to study the regulation of ergosterol biosynthesis. Transformants with 10 or 20 fold increase in acetoacetyl CoA thiolase activity show the same ergosterol (aerobiosis) and squalene (anaerobiosis) contents as the wild type strains, confirming the presence of limiting steps in the conversion of acetoacetyl CoA into sterols. Measurement of mevalonate and phosphomevalonate synthesis shows that mevalonate kinase could be repressed in transformed strains. |
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