| 生物合成薯蓣皂素的途径设计及关键酶分析 |
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| 引用本文： | 孙忠义,赵鹏,葛喜珍,田平芳. 生物合成薯蓣皂素的途径设计及关键酶分析[J]. 生物工程学报, 2021, 37(4): 1178-1188 |
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| 作者姓名： | 孙忠义 赵鹏 葛喜珍 田平芳 |
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| 作者单位： | 1 北京化工大学 生命科学与技术学院，北京 100029;2 北京联合大学 生物化学工程学院，北京 100023 |
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| 基金项目： | 国家重点研发计划 (No. 2018YFA0901800)，河北省重点研发计划 (Nos. 20322501D, 19226509D)，北京市教育委员会科研计划-北京市自然科学基金项目 (No. KZ201911417049) 资助。 |
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| 摘 要： | 薯蓣皂素是一种天然甾体皂苷元,可作为数百种类固醇药物的前体,具有重要药用价值.目前工业生产薯蓣皂素主要依赖化学提取法,因此该法依赖植物材料和耕地且对环境有害.随着代谢工程和合成生物学的发展,生物合成法受到广泛关注.文中综述了生物合成薯蓣皂素的代谢途径和关键酶,并在酿酒酵母Saccharomyces cerevisiae...
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| 关 键 词： | 薯蓣皂素 生物合成途径 关键酶 异源表达 |
| 收稿时间： | 2020-06-28 |
Pathway design and key enzyme analysis of diosgenin biosynthesis |
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| Zhongyi Sun,Peng Zhao,Xizhen Ge,Pingfang Tian. Pathway design and key enzyme analysis of diosgenin biosynthesis[J]. Chinese journal of biotechnology, 2021, 37(4): 1178-1188 |
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| Authors: | Zhongyi Sun Peng Zhao Xizhen Ge Pingfang Tian |
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| Affiliation: | 1 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China;2 College of Biochemical Engineering, Beijing Union University, Beijing 100023, China |
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| Abstract: | As a naturally occurring steroid sapogenin, diosgenin acts as the precursor of hundreds of steroid medicines, and thereby has important medicinal value. Currently, industrial production of diosgenin relies primarily on chemical extraction from plant materials. Clearly, this strategy shows drawbacks of excessive reliance on plant materials and farmland as well as environment pollution. Due to development of metabolic engineering and synthetic biology, bio-production of diosgenin has garnered plenty of attention. Although the biosynthetic pathways of diosgenin have not been completely identified, in this review, we outline the identified biosynthetic pathways and key enzymes. In particular, we suggest heterologous biosynthesis of diosgenin in Saccharomyces cerevisiae. Overall, this review aims to provide valuable insights for future complete biosynthesis of diosgenin. |
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| Keywords: | diosgenin biosynthetic pathway key enzymes heterologous expression |
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