用于药物评价的体外心脏组织模型研究进展

候选药物对心脏的毒副作用是其在开发过程中被淘汰的重要原因之一.传统药物评价所采用的动物模型存在种属差异、成本高、效率低等缺陷.因此,近年来随着干细胞和生物打印等技术的快速发展,体外心脏组织模型的构建受到了越来越多地关注.本文追踪体外心脏模型构建的起源与发展,综述模型所利用的心肌细胞来源以及二维、三维模型构建的相关技术与方法,着重阐述心肌组织模型血管化的重要性及研究进展,并对该领域未来的发展方向进行展望,以期为体外心肌组织模型在药物评价方面的研究和应用提供新思路.

Research Progress in Cardiac Tissue Model for In vitro Drug Evaluation

Drug-induced cardiotoxicity is one of the important causes of the failure in drug development. The traditional animal models for drug evaluation have many defects such as species differences, high cost, and low efficiency. It is crucial for drug development to construct more economical and efficient toxicity evaluation models which can accurately simulate the physiological characteristics of human heart. In recent years, with the rapid development of stem cell and bioprinting technologies, the construction of in vitro cardiac tissue model has received more and more attention. In this review, the origin and development of in vitro cardiac model construction are overviewed. The cell sources of cardiomyocyte and the relative techniques and methods for model construction are comprehensively reviewed. Combining with the construction technologies such as bio-printing and microfluidics,the importance and research progress of vascularized cardiac tissue model are highlighted. Finally, the future perspectives and challenges of this field are discussed in order to provide new ideas for the research and application of in vitro cardiac tissue model in drug evaluation.