The narQP genes for a two-component regulatory system from the deep-sea bacterium Shewanella violacea DSS12. |
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| Authors: | Hideyuki Tamegai Sayaka Chikuma Masami Ishii Kaoru Nakasone Chiaki Kato |
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| Institution: | Department of Chemistry, College of Humanities and Sciences, Nihon University, Tokyo, Japan. htamegai@chs.nihon-u.ac.jp |
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| Abstract: | Shewanella violacea DSS12 is facultative piezophile isolated from the deep-sea. The expression of cydDC genes (required for d-type cytochrome maturation) of the organism is regulated by hydrostatic pressure. In this study, we analyzed the nucleotide sequence upstream of cydDC in detail and found that there are putative binding sites for the NarL protein which is part of a two-component regulatory system also containing the sensor protein NarX. Furthermore, we identified the narQP genes (homologues of narXL) from S. violacea DSS12 and demonstrated the heterologous expression of narP in Escherichia coli. These results will be helpful in examining pressure regulation of gene expression in S. violacea at the molecular level. |
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