Capacity for aluminium uptake depends on brefeldin A-sensitive membrane traffic in tobacco (Nicotiana tabacum L. cv. BY-2) cells |
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| Authors: | V. A. Vitorello A. Haug |
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| Affiliation: | CENA, Universidade de S?o Paulo, Cx. Postal 96, Piracicaba, 13416, SP, Brazil, BR
Department of Microbiology, Michigan State University, East Lansing, MI 48824, USA Fax: +1-517-3535598, US
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| Abstract: | The relationship between cellular growth and aluminium (Al) uptake was examined by applying brefeldin A, a vesicle transport inhibitor, to cultured tobacco (Nicotiana tabacum L. cv. BY-2) cells. Cultured cells almost completely lost the capacity for Al uptake when pre-incubated for 1–3 h in a minimal medium. Pre-incubation also diminished subsequent growth in a culture medium. However, competency for Al uptake (20 μm, pH 4.5) was sustained in a dose-dependent and reversible manner when cells were treated with brefeldin A (10 μm), an inhibitor of Golgi-mediated secretion, prior to and during the incubation in minimal medium. Received: 24 September 1998 / Revision received: 24 November 1998 / Accepted: 5 December 1998 |
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| Keywords: | Aluminium uptake Brefeldin A Cell culture Nicotiana tabacum |
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