Automated maintenance of embryonic stem cell cultures |
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Authors: | Terstegge Stefanie Laufenberg Iris Pochert Jörg Schenk Sabine Itskovitz-Eldor Joseph Endl Elmar Brüstle Oliver |
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Affiliation: | Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn and Hertie Foundation, Bonn, Germany. |
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Abstract: | Embryonic stem cell (ESC) technology provides attractive perspectives for generating unlimited numbers of somatic cells for disease modeling and compound screening. A key prerequisite for these industrial applications are standardized and automated systems suitable for stem cell processing. Here we demonstrate that mouse and human ESC propagated by automated culture maintain their mean specific growth rates, their capacity for multi-germlayer differentiation, and the expression of the pluripotency-associated markers SSEA-1/Oct-4 and Tra-1-60/Tra-1-81/Oct-4, respectively. The feasibility of ESC culture automation may greatly facilitate the use of this versatile cell source for a variety of biomedical applications. |
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Keywords: | embryonic stem cell cell culture standardization cell culture automation cell growth pluripotency screening |
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