Influence of carbon source and inoculum type on anaerobic biomass adhesion on polyurethane foam in reactors fed with acid mine drainage |
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| Authors: | Rodriguez Renata P Zaiat Marcelo |
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| Institution: | a Instituto de Ciência e Tecnologia (ICT), Universidade Federal de Alfenas (UNIFAL), campus Poços de Caldas, Rodovia José Aurélio Vilela, 11.999, 37715-400 Poços de Caldas, MG, Brazil
b Laboratório de Processos Biológicos (LPB), Departamento de Hidráulica e Saneamento, Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Engenharia Ambiental-Bloco 4-F, Av. João Dagnone, 1100, Santa Angelina, 13.563-120 São Carlos, SP, Brazil |
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| Abstract: | This paper analyzes the influence of carbon source and inoculum origin on the dynamics of biomass adhesion to an inert support in anaerobic reactors fed with acid mine drainage. Formic acid, lactic acid and ethanol were used as carbon sources. Two different inocula were evaluated: one taken from an UASB reactor and other from the sediment of a uranium mine. The values of average colonization rates and the maximum biomass concentration (Cmax) were inversely proportional to the number of carbon atoms in each substrate. The highest Cmax value (0.35 g TVS g−1 foam) was observed with formic acid and anaerobic sludge as inoculum. Maximum colonization rates (vmax) were strongly influenced by the type of inoculum when ethanol and lactic acid were used. For both carbon sources, the use of mine sediment as inoculum resulted in a vmax of 0.013 g TVS g−1 foam day−1, whereas 0.024 g TVS g−1 foam day−1 was achieved with anaerobic sludge. |
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| Keywords: | Acid mine drainage Sulfate Biomass adhesion Biofilm Polyurethane foam |
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