Microtubule-associated STOP protein deletion triggers restricted changes in dopaminergic neurotransmission |
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Authors: | Bouvrais-Veret Caroline Weiss Stéphanie Hanoun Naima Andrieux Annie Schweitzer Annie Job Didier Hamon Michel Giros Bruno Martres Marie-Pascale |
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Institution: | Inserm, U513, Créteil, France; Univ Paris 12, Facultéde Médecine Henri Mondor, Créteil, France; Inserm, U677, Paris, France; Univ Pierre et Marie Curie-Paris 6, Facultéde Médecine, site Pitié-Salpêtrière, Paris, France; Inserm, U366, CEA, Grenoble, France |
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Abstract: | The microtubule-associated stable tubule only polypeptide (STOP) protein plays a key-role in neuron architecture and synaptic plasticity. Recent studies suggest that schizophrenia is associated with alterations in the synaptic connectivity. Mice invalidated for the STOP gene display phenotype reminiscent of some schizophrenic-like symptoms, such as behavioral disturbances, dopamine (DA) hyper-reactivity, and possible hypoglutamatergia, partly improved by antipsychotic treatment. In the present work, we examined potential alterations in some DAergic key proteins and behaviors in STOP knockout mice. Whereas the densities of the DA transporter, the vesicular monoamine transporter and the D1 receptor were not modified, the densities of the D2 and D3 receptors were decreased in some DAergic regions in mutant versus wild-type mice. Endogenous DA levels were selectively decreased in DAergic terminals areas, although the in vivo DA synthesis was diminished both in cell bodies and terminal areas. The DA uptake was decreased in accumbic synaptosomes, but not significantly altered in striatal synaptosomes. Finally, STOP knockout mice were hypersensitive to acute and subchronic locomotor effects of cocaine, although the drug equally inhibited DA uptake in mutant and wild-type mice. Altogether, these data showed that deletion of the ubiquitous STOP protein elicited restricted alterations in DAergic neurotransmission, preferentially in the meso-limbic pathway. |
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Keywords: | cocaine cytoskeleton dopaminergic transporter and receptors schizophrenia synaptosomes tyrosine hydroxylase |
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