An Embedding Method for Monolayer Cell Cultures for Light and Electron Microscopy

Plastic containers are widely used for monolayer cell culture. In situ embedding, the obvious method of choice for subsequent ultrastructural study, has been achieved by Brinkley et al. (1967) using Epon as a final embedding medium and water soluble acrylates as intermediates. Although the results are satisfactory, the method has two drawbacks: firstly, the water soluble acrylates are difficult to get, and secondly, removal of the plastic container is possible only with blocks already cut off for electron microscopy.