Apparent nuclear localization of unoccupied receptors for 1,25-dihydroxyvitamin D3 |
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Authors: | M R Walters W Hunziker A W Norman |
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Affiliation: | Department of Biochemistry University of California Riverside, California 92521 USA |
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Abstract: | Previous studies have demonstrated that unoccupied 1,25-dihydroxyvitamin D3 receptors are associated with crude chromatin under hypotonic conditions . The data presented herein show that unoccupied 1,25-dihydroxyvitamin D3 receptors appear to be associated with chromatin prior to solubilization by dilution/homogenization in both high and low salt buffers. Additionally the unoccupied receptors are recovered nearly quantitatively from purified nuclei. These results suggest that unoccupied 1,25-dihydroxyitamin D3 receptors may be localized within nuclei . |
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Keywords: | TD 10 mM Tris, 1.0 mM dithiothreitol TED 10 mM Tris, 1.5 mM EDTA, 1.0 mM dithiothreitol KTED TED, 0.3 M KCl 20% tissue weight/vol 0.2 g tissue per ml homogenate |
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